Dr Hanafy Mahmoud Ismail

Post Doctoral Research Associate

Dr. Ismail received his BSc degree in pesticide chemistry and toxicology from Alexandria University (AU), Egypt. After graduation he lectured in chemistry and pesticides modules to undergraduate and postgraduate students. In 2005 he obtained his master degree from the AU for research on insectcide analysis and formulation. In 2007, he moved to the Liverpool School of Tropical Medicine to work on molecular aspects of insectcide resistance.

Dr. Ismail research is focussed on understanding interactions of small molecules (drugs and insecticides) and their targets in parasites and insect vectors to develop better tools to control vector-borne diseases. Through his Ph.D. program (2007-2011) funded by LSTM/IVCC he developed novel chemical probes resembling pyrethroid insectcides and used them to understand the molecular mechanisms of pyrethroid resistance. The research used protein labeling chemistry to analyze enzyme function utilizing activity-based protein profiling (ABP) probes and led to the discovery of the "pyrethrome" – an enzyme network associated with pyrethroid metabolism (Ismail et al. 2013 PNAS). Recently he used the ABP approach to determine the mode of action of antimalarial drugs in Plasmodium falciparum. This work identified for the first time the proteins in the parasites that were affected by antimalarial drugs including Artemisinin (Ismail et al. 2016 PNAS) and Trioxolanes (Ismail et al. 2016 Angew. Chem. Int. Ed).

In addition to basic research activities Dr. Ismail has experience in translational and applied tropical medicine. He worked for 18 months as a Program Manager for a new venture between the Ministry of Health in the Kingdom of Saudi Arabia (KSA), Liverpool School of Tropical Medicine (LSTM) and the Innovative Vector Control Consortium (IVCC) to increase capacity to control malaria and dengue.Dr. Ismail returned to the LSTM Vector Biology Department in February 2013 to work with Dr. Mark Paine, on developing and optimizing simple field tests for DDT and organophosphate analysis used in Visceral Leishmaniasis and Malaria control operations in India. His honorary position “2013/2014” as a visiting research fellow, Faculty of Biological Sciences, University of Leeds has enabled Ismail to maintain a close collaboration with Prof. Paul Milner’s laboratory and has resulted in collaborative grant applications to explore the application of Adhiron technology in the development of insecticide monitoring tools.

Current grants

£48,140 Medical Research Council CiC Award. New tool to predict insecticide resistance: development of activity based probes to identify metabolic resistance genes in tropical disease transmitting insects   1/08/2016 – 31/07/18 (PI Mark Paine, Co-I, Ismail H, Rowland M, O’Neill P, Austin, J, David, M)

Completed research support

£27,955    Medical Research Council CiC Award. Development of Adhiron based insecticide-monitoring tools. 1/08/14 – 31/07/15 (PI Paine, Co-PI Ismail, H., O’Neill, P, Millner, P.)

£5,500   Research Development Fund, LSTM. Development of preemptive diagnostic probes to predict insecticide resistance liabilities in neglected tropical diseases vectors. PI Hanafy Ismail, Co PIs Mark Paine and Steve Torr (LSTM). (April 2015).

Publications

  • Selected Publications

    Corine Ngufor, Raphael N’Guessan, Josias Fagbohoun, Damien Todjinou, Abibath Odjo, David Malone, Hanafy Ismail, Martin Akogbeto and Mark Rowland (2016).Efficacy of the Olyset Duo net against insecticide-resistant mosquito vectors of malaria Science Translational Medicine 14 Sep 2016:Vol. 8, Issue 356, pp. 356
     
    Ismail, H. M, Victoria E. Barton, Matthew Panchana, Sitthivut Charoensutthivarakul, Giancarlo A. Biagini, Stephen A. Ward, and Paul M. O’Neill. Click Chemistry Based Proteomics highlights a common malaria parasite protein alkylation signature between synthetic 1,2,4-Trioxolane Antimalarials and the Artemisinins. Angewandte Chemie International Edition. Volume 55, Issue 22, May 23, 2016, Pages 6401–6405
     
    Ismail, H. M.; Barton, V.; Phanchana, M.; Charoensutthivarakul, S.; Wong, M. H.; Hemingway, J.; Biagini, G. A.; O’Neill, P. M.; Ward, S. A., Artemisinin activity-based probes identify multiple molecular targets within the asexual stage of the malaria parasites Plasmodium falciparum 3D7. Proceedings of the National Academy of Sciences 2016, 113, 2080-2085.

    Recommended in F1000 Prime “very good paper” https://f1000.com/prime/726131980

     

    Ismail, H. M.; Kumar, V.; Singh, R. P.; Williams, C.; Shivam, P.; Ghosh, A.; Deb, R.; Foster, G. M.; Hemingway, J.; Coleman, M.; Coleman, M.; Das, P.; Paine, M. J., Development of a Simple Dipstick Assay for Operational Monitoring of DDT. PLoS neglected tropical diseases 2016, 10, e0004324-e0004324.

    Coleman, M.; Foster, G. M.; Deb, R.; Singh, R. P.; Ismail, H. M.; Shivam, P.; Ghosh, A. K.; Dunkley, S.; Kumar, V.; Coleman, M., DDT-based indoor residual spraying suboptimal for visceral leishmaniasis elimination in India. Proceedings of the National Academy of Sciences 2015, 112, 8573-8578.

    Toé, K. H.; Jones, C. M.; N’Fale, S.; Ismail, H.; Dabiré, R. K.; Ranson, H., Increased pyrethroid resistance in malaria vectors and decreased bed net effectiveness, Burkina Faso. Emerging infectious diseases 2014, 20, 1691-1696.

    Russell, T. L.; Morgan, J. C.; Ismail, H.; Kaur, H.; Eggelte, T.; Oladepo, F.; Amon, J.; Hemingway, J.; Iata, H.; Paine, M., Evaluating the feasibility of using insecticide quantification kits (IQK) for estimating cyanopyrethroid levels for indoor residual spraying in Vanuatu. Malar J 2014, 13, 10.1186.

    Riveron, J. M.; Yunta, C.; Ibrahim, S. S.; Djouaka, R.; Irving, H.; Menze, B. D.; Ismail, H. M.; Hemingway, J.; Ranson, H.; Albert, A., A single mutation in the GSTe2 gene allows tracking of metabolically based insecticide resistance in a major malaria vector. Genome Biol 2014, 15, R27.

    Ismail, H. M.; O’Neill, P. M.; Hong, D. W.; Finn, R. D.; Henderson, C. J.; Wright, A. T.; Cravatt, B. F.; Hemingway, J.; Paine, M. J., Pyrethroid activity-based probes for profiling cytochrome P450 activities associated with insecticide interactions. Proceedings of the National Academy of Sciences 2013, 110, 19766-19771. 

    David, J. -P.; Ismail, H. M.; Chandor-Proust, A.; Paine, M. J. I., Role of cytochrome P450s in insecticide resistance: impact on the control of mosquito-borne diseases and use of insecticides on Earth. Philosophical Transactions of the Royal Society of London B: Biological Sciences 2013, 368, 20120429.