Mr Daniel Southern

Research Technician/Tsetse Technician

Daniel Southern graduated from Liverpool John Moores University in 2008 with a BSc in Biochemistry and Microbiology and obtained an MSc in Molecular Biology of Parasites and Vectors at Liverpool School of Tropical Medicine in 2012. He is currently working in the team of Dr Alvaro Acosta Serrano at Liverpool School of Tropical Medicine examining Trypanosome and Tsetse fly interactions. 

Human and Animal African trypanosomiasis

Glossina morsitans morsitans feeding on horse blood through a silicon membrane

Identification of genes linked to trypanosome establishment and development inside the tsetse (Glossina morsitans morsitans) vector. I work with gene knockdown in insecta using dsRNA feeding and injection techniques. The degree of knockdown is further quantitated using real time PCR and western blotting assays. This data is used alongside observed fly knockdown phenotypes affecting vectorial capacity and/or fly biology. We characterise key genes that affect host and/or parasite interactions as this could possibly lead to novel methods of vector control. To help maintain our groups’ extensive tsetse research portfolio, I help maintain our tsetse colony of >10,000 flies, which is the largest breeding colony in the UK.

 

 

 

Glossina morsitans morsitans feeding on a human arm

 

 

 

*Tsetse photos are used with author’s permission: http://www.raywilsonbirdphotography.co.uk/Galleries/Invertebrates/vector...

Images cannot be copied, downloaded or used without full permission granted by photographer Ray Wilson.

 

 

 

 

LSTM breeding colony of >10,000 Glossina morsitans morsitans (credit Lee Haines).