Professor Alister Craig

Dean of Biological Sciences

Areas of interest

Cytoadherence in malaria; ex vivo models of adhesion; rational design of anti-adhesive compounds using mutagenesis and structural information; signalling pathways in malaria; functional genomics including mapping functional differences in cytoadherence using proteomic analysis; analysis of adherent parasites in patient samples.

Background

Alister Craig graduated in Genetics from Edinburgh University in 1981 and obtained his PhD in Molecular Biology from Leicester University in 1984.  He spent the next two years as an EMBO Fellow at EMBL in Heidelberg followed by two years as an ICRF Fellow in London working on developing techniques for genome analysis.  He subsequently worked for ten years at the Institute of Molecular Medicine in Oxford on malaria before joining the Liverpool School of Tropical Medicine in 1999.

Research

One aspect of malaria biology that has been associated with severity of disease has been the ability of erythrocytes infected with Plasmodium falciparum to adhere to the endothelial cells lining the small blood vessels (and also within the placenta).  Several endothelial receptors are able to mediate this binding, but studies on patient isolates have identified a subset of these are being important in the field.  Our research has focussed on one of the major receptors, intercellular adhesion molecule 1 (ICAM-1) and also uses live endothelium as a model of the interactions taking place in vivo, as recent studies have indicated that disease severity may be linked to the ability of parasites to adhere to multiple receptors.  The group's work has extended into an analysis of post-adhesive effects on both the parasite and the host endothelium.

As well as wishing to understand the molecular processes underpinning sequestration in malaria, we are also carrying out work on clinical correlation of specific types of adhesion with severe disease and the differential distribution of variant populations of parasites in the body due to receptor tropism.  Our main goal is an understanding of the pathology of adhesion-based pathology in malaria and, thereby, the development of novel anti-disease therapeutics.

Other relevant expertise

Member of the Wellcome Trust Expert Review Group (Pathogen Biology and Disease Transmission)

Publications

  • Selected Publications

    Dumizulu L. Tembo, Benjamin Nyoni, Rekah V. Murikoli , Mavuto Mukaka, Danny A. Milner, Matthew Berriman, Stephen J. Rogerson, Terrie E. Taylor, Malcolm E. Molyneux, Wilson L. Mandala, Alister G. Craig and Jacqui Montgomery(2014) Differential PfEMP1 Expression is Associated with Cerebral Malaria Pathology. Plos Pathog., 10(12): e1004537 

    Valentina Barrera, Paul S Hiscott, Alister G Craig, Valerie A White, Milner DA, Nicholas AV Beare, Ian JC MacCormick, Steve Kamiza, Terrie E Taylor, Malcolm E Molyneux, Simon P Harding (2014) Severity of Retinopathy Parallels the Degree of Parasite Sequestration in Eye and Brain in Malawian Children with Fatal Cerebral Malaria. J. Infect. Dis., 210 (12): 1991-2000 

    Christopher A. Moxon, Ngawina V. Chisala, Samuel C. Wassmer, Terrie E. Taylor, Karl B. Seydel, Malcolm E. Molyneux, Brian Faragher, Neil Kennedy, Cheng-Hock Toh, Alister G. Craig, Robert S. Heyderman (2014) Persistent endothelial activation and inflammation after Plasmodium falciparum infection in Malawian children. J. Infect. Dis. 209(4): 610-615 

    Christopher Alan Moxon, Samuel Crocodile Wassmer, Danny Arnold Milner, Ngawina V Chisala, Terrie Taylor, Karl B Seydel, Malcolm E Molyneux, Brian Faragher, Charles T. Esmon, Colin Downey, Cheng-Hock Toh, Alister G. Craig, and Robert Simon Heyderman (2013) Loss of endothelial protein C receptors links coagulation and inflammation to parasite sequestration in cerebral malaria in African children. Blood 122(5):842-851 

    Alan Brown, Louise Turner, Stig Christoffersen, K.A. Andrews, Tadge Szestak, Yuguang Zhao, S. Larsen, Alister G. Craig and Matthew K. Higgins (2013) Molecular architecture of a complex between an adhesion protein from the malaria parasite and intercellular adhesion molecule 1. J. Biol. Chem., 288 (8): 5992-6003 

    Anja Bengtsson, Louise Joergensen, Thomas S Rask, Rebecca W Olsen, Marianne A Andersen, Louise Turner, Thor G Theander, Lars Hviid, Matthew K Higgins, Alister Craig, Alan Brown and Anja TR Jensen (2013) A novel domain cassette identifies Plasmodium falciparum PfEMP1 proteins that bind ICAM‑1 and is a target of cross-reactive, adhesion-inhibitory antibodies. J. Immunol, 190 (1): 240-249 

    Lucy B. Ochola, Bethsheba R. Siddondo, Harold Ocholla, Siana Nyka, Eva N. Kimani, Thomas N. Williams, Johnstone O. Makale, Anne Liljander, Britta C. Urban, Pete C. Bull, Tadge Szestak, Kevin Marsh and Alister G. Craig(2011) Specific receptor usage in Plasmodium falciparum cytoadherence is associated with disease outcome. PLoS ONE 6(3): e14741

    Samuel Wassmer, Christopher Moxon, Terrie Taylor, Georges Grau, Malcolm Molyneux  and Alister Craig (2011) Vascular endothelial cells cultured from patients with cerebral or uncomplicated malaria exhibit differential reactivity to TNF. Cell. Microbiol. 13(2): 198-209